ree radical, very strong reactant, may injury to tissue react on the DNA, cell membrane
lipid and protein.
It was known that free radical may play an important role in the pathogenesis of varying
disease such as respiratory tract disease, epidermal carcinoma, psoriasis, rheumatoid arthritis
and autoimmune disease, also aging process.
We studied to assess the antioxidant status of red cells in human diabectics by measuring
activities of celluar antioxidant enzymes (SOD,GSHPX,Catalase),G6PDH and total glutatione
level.
The result were summarized as follows :
1) The superoxide dismutase altivity of red cells in diabetics (2.204±0.711 U/mg Hb) was
significantly lower than in normal control group (3.070±0.721 U/mg Hb) (p<0.05).
2) The glucose 6-phosphate dehydrogenase (G6PDH) activity of red cells in diabetics(0.648
±0.241 U/mg Hb) was significantly higher than in normal control group (0.456±0.190 U/mg
Hb) (p<0.05).
3) The superoxide dismutase activity of red cells in diabetics over 10 years duration 91.96
±0.92 U/mg Hb) was significantly lower than in normal control group (3.07±0.72 U/mg
Hb)(p<0.05), also glutathione peroxidase activity of red cells in diabetics over 10 years
duration (0.74±0.17 U/mg Hb) was significantly lower than in normal control group (1.81±
0.48 U/mg Hb), in diabetics below 5 years duration (1.26±0.38 U/mg Hb) and in diabetics 5
to 10 years duration (1.45±0.42 U/mg Hb) (p<0.01).
4) The catalase activity of red cell in diabetics associated with retinopathy (2.93 0.51
U/mg Hb) was significantly lower than in D.M. without retiopathy (4.01 0.28 U/mg Hb)
(p<0.01).
5) The glutathione peroxidase activity of red cell in diabetics associated with retinopathy
(0.74±0.27 U/mg Hb) was significantly lower than in D.M. Without retinopathy (1.47±0.54
U/mg Hb) (p<0.05) and in normal control group (1.81±0.48 U/mg Hb) (p<0.01).
6) The glutathione peroxidase activity of red cells in diabetics associated with nephropathy
(1.10±0.39 U/mg Hb) was significantly lower than in normal control group (1.81±0.48 U/mg
Hb) (p<0.05), also total glutathione levels in D.M. With nephropathy (10.61±4.48 nM/mg Hb)
was significanthy lower than in D.M. without nephropathy (15.90±4.21 nM/mg Hb) (p<0.05).
These results suggest that tissue antioxidant status may be an important factor in the
etiology and complication of diabetes mellitus, but it is not clear wheather tissue antioxidant
system was affected, and additional study will be needed for a full understanding of the role
that free radical play in the antioxidant defense systems.
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